Protocol: Marine Invertebrate Sequencing 2023
DOI: 10.1000/proto.126
DNA was extracted from marine invertebrate tissue samples using the CTAB method. The quality and quantity of the extracted DNA were assessed using a Qubit Fluorometer and an Agilent 2100 Bioanalyzer. Libraries were prepared using the TruSeq DNA PCR-Free Library Prep Kit. Sequencing was conducted on a PacBio Sequel II system, generating HiFi reads. The sequencing data were processed using SMRT Link software for read quality filtering and genome assembly.