Genomic DNA was extracted from avian blood samples using the Qiagen DNeasy Blood & Tissue Kit. The extracted DNA was quantified using a Qubit Fluorometer and quality checked using a Nanodrop spectrophotometer. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit, following the manufacturer's instructions. Sequencing was performed on an Illumina NovaSeq 6000 platform with paired-end reads of 150 bp. Data analysis included quality control using FastQC, read alignment with BWA, and variant calling with GATK.