Protocol: Avian Genome Sequencing 2022

Genomic DNA was extracted from avian blood samples using the Qiagen DNeasy Blood & Tissue Kit. The extracted DNA was quantified using a Qubit Fluorometer and quality checked using a Nanodrop spectrophotometer. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit, following the manufacturer's instructions. Sequencing was performed on an Illumina NovaSeq 6000 platform with paired-end reads of 150 bp. Data analysis included quality control using FastQC, read alignment with BWA, and variant calling with GATK.
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This work has been supported by the Wellcome Sanger Insitute and Arizona State University.